Oligo Pools
Scalable oligo pools for high-throughput applications—from hundreds to millions of sequences—supporting CRISPR, gene synthesis, and synthetic biology workflows, with cost-effective pricing at scale.
Watsonbio Sciences proudly serves as the exclusive distributor of Dynegene https://www.dynegene.com/en/, a global leader in innovative DNA and RNA synthesis technologies. Dynegene’s state-of-the-art platforms deliver high-quality gene synthesis, oligo pools, and sgRNA libraries that power breakthroughs in genomics, synthetic biology, and molecular diagnostics.
Cost effective high-throughput DNA Synthesis
Nucleic acid synthesis is the backbone of innovation in life sciences—powering breakthroughs across biomedicine, genomics, synthetic biology, and beyond. As the demand for faster, more scalable, and cost-effective solutions grows, ultra-high-throughput nucleic acid synthesis has emerged as a game-changing technology with transformative potential across industries. At Watsonbio, we are redefining what’s possible in next-generation nucleic acid synthesis. We’ve broken through the limitations of traditional methods with our proprietary DYHOW platform—a next-generation, ultra-high-throughput DNA synthesis system built from the ground up with independent intellectual property.
Our latest innovation, DYHOW-3B, delivers unprecedented performance:
- Synthesis of up to 4.35 million unique custom DNA sequences
- Maximum oligo length of 350 nucleotides
- Remarkably high accuracy and uniformity
- Significantly reduced synthesis costs and turnaround time
With DYHOW-3B, Watsonbio empowers rapid innovation across synthetic biology, medicine, agriculture, food science, and more—solving real-world challenges from research to industrial production.
Workflows
Highly Uniform and Accurate Synthesis
NGS sequencing verification of oligo pools with identical sequences synthesized separately by Watsonbio and a competitor revealed superior uniformity in Watsonbio’s synthesized oligo pools.
Applications
Gene Editing
Oligo pools serve as a targeted gene screening tool to identify molecular and therapeutic targets. They are widely applied in tumor diagnostics, agricultural bio-breeding, and microbial engineering, enabling rapid functional genomics at scale.
Fluorescence In Situ Hybridization
High-throughput oligo synthesis enables the rapid development of FISH probes and spatial transcriptomics platforms such as MerFISH. These tools support spatially resolved, single-cell gene expression profiling across complex tissue samples.
Mutant Library
Thousands of sequence variants can be synthesized simultaneously to identify key structural and functional residues. This accelerates protein engineering, metabolic engineering, antibody drug development, and the optimization of industrial enzymes.
Targeted Sequencing
By designing specific probe and primer sequences and producing them on a high-throughput synthesis platform, the target genomic region can be effectively enriched using highly optimized capture reagents, substantially reducing sequencing cost and turnaround time.
Gene Synthesis
Long genes are typically assembled from short oligo fragments. High-throughput oligo pool synthesis enables the parallel manufacture of large numbers of gene sequences, powering applications such as mRNA vaccine antigen screening and large-scale gene library construction.
Data Storage
DNA possesses inherent properties of high density, chemical stability, and extraordinary durability, making it a compelling medium for long-term information archiving. Oligo pool synthesis platforms make large-scale DNA data storage practically accessible.
Application Example
Oligo pool synthesis is the foundation of library construction. To build a successful library, strict control is necessary throughout the synthesis process. Leveraging the high-throughput DNA synthesis platform developed by Watsonbio, we constructed a library containing 63,950 sgRNA sequences. NGS verification demonstrated high coverage and uniformity of the sgRNA library.
Analysis of NGS data from four batches of oligo pool-constructed libraries revealed high coverage, uniform distribution, and excellent stability across batches of the sgRNA libraries.