Multiplex miRNA Assay

SimPlex-miR™ : Simple multiplex miRNA

• Innovation: Multiple miRNAs in one tube within 20 min without PCR amplification.

• High Fidelity miRNAs Profiles: Solve the bottle neck of miRNAs biomarker assay, including batch variations of isolation yield, bias of miRNA labelling/ligation, and bias of PCR amplification from GC contents and annealing temperature.

• Applications: Liquid biopsy of cancer early diagnostics, drug selection and R&D for new therapeutics.

SimPlex-miR™ overcomes the bias from PCR amplification (Company Q) and redundant operations and long/high temperature hybridization (Company T)

Challenges - Limitations of Current PCR Based miRNA Assay

Mass of total miRNAs is less than 0.1% from sera, and consists of 13,000 kinds of different sequences.

1. Batch Variations of miRNA Isolation
   • Consistency from different measurement and different group
2. Biases in RNA Ligation & PCR Amplification
   • Significant biases introduced by RNA ligation lead to inaccurate miRNA quantification by 1000 folds.
   • Respective miRNA G/C content greatly influences rates of cDNA synthesis and is also responsible for template-specific preferences in PCR amplification.

Raabe et al., Nucleic Acids Research, 2014

Innovations - SimPlex-miR™ Providing High Fidelity Profiles

Accuracy from SimPlex: 20 min. mix/hybridization + 1 min analysis.

• Short hybridization time reduces the possibility of miRNA degradation and non-specific adsorption.
• Multiplex miRNA assay and analysis methodology solves the batch variations.

Summary of SimPlex-miR™

Applicable to other type of linear RNA, such as IncRNA, mRNA

Cost reduced to 5-10％ of other miRNAs assay

High Fidelity miRNAs Profiling

• No PCR bias
• No miRNA ligation bias
• No miRNA isolation batch variations
• Short assay time, less miRNA degradation and nonspecific adsorption

Please contact info@watsonbio.com for details