Antibody Sequencing

Watsonbio provides convenient, rapid, reliable, and customized sequencing services for your valuable monoclonal antibodies. Our platform supports sequencing from any antibody-producing cell line, with services tailored to meet your specific needs — whether for quality control, intellectual property protection, antibody engineering, or therapeutic development. We combine speed with precision to support your downstream research and commercialization goals.

Features

• Convenient Sample Handling: Our proprietary enzyme technology enables RNA lysis at room temperature - NO DRY ICE needed. We provide both the RNA lysis buffer and a FedEx shipping label to simplify your logistics.
• Unmatched Accuracy: Bi-directional sequencing of multiple independent clones ensures highly accurate and reliable results.
• Cost-Effective & Fast: Enjoy competitive pricing without compromising on quality. We offer rapid turnaround to meet your project timelines.
• Comprehensive Reporting: Each project includes a detailed report featuring annotated antibody isotype, DNA and protein sequences, and identified CDRs.

Sample Requirements

We accept a variety of cell types, including hybridoma cells, B cells, and single B cells.

• Minimum cell count: > 2 × 10⁶ cells
• Collection: Add 500 µL of the provided RNA lysis buffer directly to your sample and place it into the included FedEx envelope using the pre-paid shipping label.
• Supported species: Mouse, rat, rabbit, ferret, and more

Technical Summary

Service Items

• WB-1113-1: Variable Domains Sequencing – 7-10 business days Both heavy chain variable region (VH) and light chain variable region (VL) of monoclonal antibodies

• WB-1113-2: Variable Domains Plus Sequencing – 10-15 business days Both heavy chain variable region (VH) and light chain variable region (VL) plus leader sequences

• WB-1113-3: Full Length Sequencing – 15-18 business days Both heavy chain variable region (VH) and light chain variable region (VL), constant domains leader sequences

• WB-1114: Recombinant Antibody Expression – 10-15 business days Order any of the sequencing services and rapid expression to confirm sequences.

  • Expression Systems: CHO or HEK293T cells
  • Deliverables:
    • Purity ≥95% by SDS-PAGE
    • Purity ≥95% by HPLC
    • Endotoxin ≤1 EU/mg
    • Certificate of Analysis (COA)
  • Turnaround Time: 2–3 weeks

• Additional Services Available: To streamline your workflow, we also offers:

  • Hybridoma antibody production
  • Antigen-antibody binding validation