360 MMP FRET Substrate IV

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This substrate has a fluorescent Trp residue in subsite Pí2 and a dinitrophenol (Dnp) quenching group at the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-Pí1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-Pí1 and Pí3 is optimized for MMP-3, while Arg is placed in Pí4 to enhance solubility. The substrate hydrolysis rate rivals or exceeds those of the corresponding natural protein substrates or other synthetic peptides. The solubility of this substrate in assay buffer exceeds the Km value for each reaction, allowing accurate determination of the kinetic parameters. Abs/Em = 280/360 nm.

Clear
Catolog No: N/A Category:

Sequence (One-Letter Code):

Dnp-PYAYWMR

 

Sequence (Three-Letter Code):

Dnp-{Pro}{Tyr}{Ala}{Tyr}{Trp}{Met}{Arg}

 

Molecular Weight:

1152.3

 

Purity:
% Peak Area By HPLC ≥ 95%

 

Salt:
TFA Salt

 

Storage:

Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.

 

Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

 

Documents:

MSDS

Amount

1 mg, 5 mg, Other