Multiplex miRNA Assay

SimPlex-miR™ : Simple multiplex miRNA

SimPlex-miR™ overcomes the bias from PCR amplification (Company Q) and redundant operations and long/high temperature hybridization (Company T)

Challenges - Limitations of Current PCR Based miRNA Assay

Mass of total miRNAs is less than 0.1% from sera, and consists of 13,000 kinds of different sequences.

I. Batch Variations of miRNA Isolation

II. Biases in RNA Ligation & PCR Amplification

Innovations - SimPlex-miR™ Providing High Fidelity Profiles

Accuracy from SimPlex: 20 min. mix/hybridization + 1 min analysis.

Summary of SimPlex-miR™

Applicable to other type of linear RNA
, such as lncRNA, mRNA

Cost reduced to 5-10% of other miRNAs assay

High Fidelity miRNAs Profiling

1. No PCR bias 2. No miRNA ligation bias 3. No miRNA isolation batch variations 4. Short assay time, less miRNA degradation and nonspecific adsorption

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