Multiplex miRNA Assay
SimPlex-miR™ : Simple multiplex miRNA
SimPlex-miR™ overcomes the bias from PCR amplification (Company Q) and redundant operations and long/high temperature hybridization (Company T)
Challenges - Limitations of Current PCR Based miRNA Assay
Mass of total miRNAs is less than 0.1% from sera, and consists of 13,000 kinds of different sequences.
Innovations - SimPlex-miR™ Providing High Fidelity Profiles
Accuracy from SimPlex: 20 min. mix/hybridization + 1 min analysis.
Summary of SimPlex-miR™
Applicable to other type of linear RNA , such as lncRNA, mRNA
Cost reduced to 5-10％ of other miRNAs assay
High Fidelity miRNAs Profiling
1. No PCR bias 2. No miRNA ligation bias 3. No miRNA isolation batch variations 4. Short assay time, less miRNA degradation and nonspecific adsorption