360 MMP FRET Substrate III

$71.00$225.00

Optimized for both 72-kDa fibroblast gelatinase(MMP 2) and 92-kDa neutrophil gelatinase(MMP 9), this peptide substrate has the fluorescent Trp residue in subsite Pí2 and the dinitrophenol (Dnp) quenching group on the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-Pí1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-Pí1 and Pí3 are optimized for MMP, while Arg is placed in Pí4 to enhance solubility. The water solubility of this peptide substrate in assay buffer exceeds the Km value for the enzyme reaction, allowing accurate determination of the kinetic parameters. Abs/Em = 280/360 nm.

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Catolog No: N/A Category:

Sequence (One-Letter Code):

Dnp-PLGMWSR

 

Sequence (Three-Letter Code):

Dnp-{Pro}{Leu}{Gly}{Met}{Trp}{Ser}{Arg}

 

Molecular Weight:

1012.1

 

Purity:
% Peak Area By HPLC ≥ 95%

 

Salt:
TFA Salt

 

Storage:

Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.

 

Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

 

Documents:

MSDS

Amount

1 mg, 5 mg, Other