Antibody Sequencing
Watsonbio provides convenient, rapid, reliable, and customized sequencing services for your valuable monoclonal antibodies. Our platform supports sequencing from any antibody-producing cell line, with services tailored to meet your specific needs — whether for quality control, intellectual property protection, antibody engineering, or therapeutic development. We combine speed with precision to support your downstream research and commercialization goals.
Features
● Convenient Sample Handling: Our proprietary enzyme technology enables RNA lysis at room temperature — NO DRY ICE needed. We provide both the RNA lysis buffer and a FedEx shipping label to simplify your logistics.
● Unmatched Accuracy: Bi-directional sequencing of multiple independent clones ensures highly accurate and reliable results.
● Cost-Effective & Fast: Enjoy competitive pricing without compromising on quality. We offer rapid turnaround to meet your project timelines.
● Comprehensive Reporting: Each project includes a detailed report featuring annotated antibody isotype, DNA and protein sequences, and identified CDRs.
Sample Requirements
We accept a variety of cell types, including hybridoma cells, B cells, and single B cells.
- Minimum cell count: > 2 × 10⁶ cells
- Collection: Add 500 µL of the provided RNA lysis buffer directly to your sample and place it into the included FedEx envelope using the pre-paid shipping label.
- Supported species: Mouse, rat, rabbit, ferret, and more
Technical Summary
Service Items
- WB-1113-1: Variable Domains Sequencing (7-10 business days)
Both heavy chain variable region (VH) and light chain variable region (VL) of monoclonal antibodies from Hybridoma
- WB-1113-2: Variable Domains Plus Sequencing (10-15 business days)
Both heavy chain variable region (VH) and light chain variable region (VL) plus leader sequences
- WB-1113-3: Full Length Sequencing (15-18 business days)
Both heavy chain variable region (VH) and light chain variable region (VL), constant domains leader sequences
- WB-1114: Recombinant Antibody Expression (10-12 business days)
Order any of the sequencing services and rapid expression to confirm sequences.
- Additional Services Available:
To streamline your workflow, we also offers:- Hybridoma antibody production
- Antigen-antibody binding validation