Custom Monoclonal Antibodies
Leveraging decades of expertise, our custom monoclonal antibody production service provides a fully integrated approach to generating high-affinity, target-specific monoclonal antibodies. This service encompasses the entire workflow, from the design and synthesis of the immunogen to the production of monoclonal antibodies in mouse or rat models. Best of all, our services are proudly made in the USA, ensuring top quality and reliability.
Our proprietary process is meticulously optimized for rapid and efficient antibody generation, utilizing advanced electrofusion technology. This approach enables the generation of an extensive repertoire of hybridoma clones, significantly increasing the likelihood of identifying antibodies with the desired specificity and affinity, even against difficult or rare epitopes.
Custom Monoclonal Antibody Development Process
Phase 1: Antigen Selection
We can use a range of macromolecules as antigens, including:
- Protein, recombinant or purified
- Synthetic peptide/modified peptide
- Haptens
- Whole cell lysate
- Nucleic acid and oligos
- Lipids
- Carbohydrate complexes
Phase 2: Antigen Selection
Once the antigen has been produced, we move into monoclonal antibody development. This phase includes a 7-step process which typically takes 14–20 weeks to complete.
| Step | Description | Time Frame |
|---|---|---|
| 1 | Immunization | 6-8 weeks |
| 2 | Screening | |
| 3 | Evaluation | |
| 4 | Fusion | 4-6 weeks |
| 5 | Expansion & Screening | |
| 6 | Subcloning | 4-6 weeks |
| 7 | Expansion & Cyripreservation |
Phase 3: Antibody Production and Purification
We provide a range of customizable options for the production and purification of monoclonal antibodies to meet the specific needs of your project.
Production options:
- Stationary flask cell culture
- Roller bottle cell culture
- Custom mouse ascites (5–200+ mice; immunodeficient mice optional)
- Concentration by ultrafiltration
Purification options:
- Protein A purification
- Protein G purification
- Protein A/G purification
- Affinity purification
- IgG purification
- IgY purification
- Low endotoxin purification
Phase 4: Cell Banking, Scale-up, and Conjugation
In addition to antigen generation and monoclonal antibody production, we can provide cell banking, manufacturing, and conjugation.
Other Antibody Development Services
- Hybridoma antibody gene sequencing
- Chimeric antibody generation
- Antibody humanization
- Affinity maturation through mammalian in vivo system
- Fast transient antibody expression in HEK293 and CHO
- Fast generation of CHO stable pool for scale-up production
- Generation of stable cell line with industrial productivity
Service Highlights
- Certified U.S. Facility Proudly made in the USA! Our Houston-based custom antibody development facility is AAALAC accredited and OLAW certified, ensuring the highest standards of care and compliance.
- Innovative Immunization Technology Our proprietary immunization technology significantly enhances the success rate of generating monoclonal antibodies with exceptional specificity and affinity.
- Guaranteed Performance We stand by our results—ELISA titers and Western blot data are guaranteed.
- Strict Confidentiality Your project is safe with us. We maintain the utmost confidentiality, ensuring no project-related information or products are disclosed to anyone but the project owner.
- Rapid Turnaround Time Receive your monoclonal antibodies 3-4 weeks faster than the industry standard, accelerating your research timelines.
- Zero Setup Fees With our high success rate, we confidently offer a zero setup fee, minimizing your risk and maximizing your investment.